Differential and opposed transcriptional effects of protein fusions containing the VP16 activation domain

FEBS Letters

Rezsohazy, René¹  Chomez, Patrick²  Picard, Jacques¹  Matis, Christelle¹ 

[1] Unit of Developmental Genetics, Université Catholique de Louvain, 73 (Boîte 82) avenue Mounier, 1200 Brussels, Belgium;The Ludwig Institute for Cancer Research, Brussels Branch, 75 (Boîte 80) avenue Hippocrate, 1200 Brussels, Belgium

关键词: VP16 activation domain;    Hoxa2 homeoprotein;    Cytomegalovirus;    Rous sarcoma virus;    Transcriptional activation;    Squelching effect;    AdML;    adenovirus-2 major late promoter;    CMV;    cytomegalovirus;    lacZ;    β-galactosidase gene from Escherichia coli;    luc;    firefly luciferase gene;    RSV;    Rous sarcoma virus;    SV40;    simian virus 40;    TK;    thymidine kinase;    VP16;    herpes simplex virion protein 16;   

DOI  :  10.1016/S0014-5793(01)02532-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Overexpression of strong transcriptional activators like herpes simplex virion protein 16 (VP16) may lead to non-specific inhibition of gene expression as a result of the titration of transcription factors. Here we report that a fusion between the homeoprotein Hoxa2 and the VP16 activation domain inhibits transcription from the strong promoter/enhancers of cytomegalovirus (CMV) and Rous sarcoma virus (RSV). A similar fusion involving a Hoxa2 mutant protein that is defective in DNA binding has no effect on the CMV promoter but increases, rather than inhibits, the RSV promoter activity. This suggests that depending on its ability to bind DNA, the VP16 activator can interact with different sets of cofactors, giving rise to distinct transcriptional effects.

【 授权许可】

Unknown