Almost the entire 5′ non‐translated region of hepatitis C virus is required for cap‐independent translation

FEBS Letters

Spaan, Willy³  Lemon, Stanley²  Bredenbeek, Peter³  Whetter, Linda²  Inchauspé, Geneviève¹  Rijnbrand, René³  van der Straaten, Tahar³ 

[1] The Lindsley F. Kimball Research institute, The New York Blood Center, New York, NY 10021, USA;Department of Medicine, The University of North Carolina at Chapel Hill, NC 27599-7030, USA;Department of Virology, Institute of Medical Microbiology, Faculty of Medicine, Leiden University, Postbus 320, 2300 AH Leiden, The Netherlands

关键词: Hepatitis-C virus;    5′ Untranslated region;    Secondary structure;    Translation;    Internal ribosome entry site;    IRES;    2A;    EMCV;    encephalomyocarditis virus;    RSV;    Rous sarcoma virus;    PCR;    polymerase chain reaction;    CAT;    chloramphenicol acetyltransferase;   

DOI  :  10.1016/0014-5793(95)00458-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate which hairpin structures within the 5′ untranslated region of hepatitis C virus (HCV) are necessary for cap-independent translation, mutants were constructed that lack one or more hairpin structures. Here we demonstrate, by constructing precisely defined hairpin deletion mutants, that with the exception of the most 5′ located hairpin structure, which on deletion shows an increase on translation, each of the predicted hairpins is found to be essential for cap-independent translation. In addition, we demonstrate that HCV 5′UTR driven translation is stimulated by poliovirus 2A^pro co-expression.

【 授权许可】

Unknown