Insights into the adaptive response of Arabidopsis thaliana to prolonged thermal stress by ribosomal profiling and RNA-Seq

BMC Plant Biology

Research Article

Peter Feist¹  Zoya Ignatova²  Radoslaw Lukoszek³ 

[1] Biochemistry, Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany;Biochemistry, Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany;Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, Hamburg, Germany;Biochemistry, Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany;Present Address: Division of Plant Sciences/Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dow Street, DD1 5EH, Dundee, UK;

关键词: Translation;    Ribosome profiling;    Transcription;    RNA-Seq;    Secondary structure;    G-quadruplexes;    Heat stress response;   

DOI  :  10.1186/s12870-016-0915-0

 received in 2016-05-17, accepted in 2016-10-05,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundEnvironmental stress puts organisms at risk and requires specific stress-tailored responses to maximize survival. Long-term exposure to stress necessitates a global reprogramming of the cellular activities at different levels of gene expression.ResultsHere, we use ribosome profiling and RNA sequencing to globally profile the adaptive response of Arabidopsis thaliana to prolonged heat stress. To adapt to long heat exposure, the expression of many genes is modulated in a coordinated manner at a transcriptional and translational level. However, a significant group of genes opposes this trend and shows mainly translational regulation. Different secondary structure elements are likely candidates to play a role in regulating translation of those genes.ConclusionsOur data also uncover on how the subunit stoichiometry of multimeric protein complexes in plastids is maintained upon heat exposure.

【 授权许可】

CC BY   
© The Author(s). 2016