Analysis of an activated ABI5 allele using a new selection method for transgenic Arabidopsis seeds

FEBS Letters

Giraudat, Jérôme¹  Parcy, François¹  Kroj, Thomas¹  Bensmihen, Sandra¹  Lambert, Guillaume¹  To, Alexandra¹ 

[1] Institut des Sciences du Végétal, UPR 2355 CNRS, 1, Av. de la terrasse, 91198 Gif-sur-Yvette Cedex, France

关键词: Abscisic acid insensitive 5;    Abscisic acid;    Transgenic seed selection;    Late embryogenesis abundant gene regulation;    VP16-AD;    VP16 activation domain;    ABA;    abscisic acid;    ABI;    abscisic acid insensitive;    LEA;    late embryogenesis abundant;    DAP;    day after pollination;    GFP;    green fluorescent protein;    ABRE;    abscisic acid responsive element;   

DOI  :  10.1016/S0014-5793(04)00148-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Arabidopsis abscisic acid (ABA) insensitive (ABI)5 transcription factor participates in the ABA-dependent induction of late embryogenesis abundant (LEA) genes in the final stages of seed development. We tested whether the VP16 transcriptional activation domain is sufficient to provide ABI5 with the ability to activate the AtEm LEA genes in vegetative tissues. We took advantage of a new transgenic seed selection assay based on green fluorescent protein (GFP) fluorescence and found that VP16-ABI5 triggered growth retardation and ABA-independent induction of AtEm1 in seedlings. These results indicate that ABI5 activation potential is a limiting step and might be a target for ABA signaling.

【 授权许可】

Unknown