Effects of gibberellic acid and abscisic acid on levels of translatable mRNA (1→3,1→4)‐β‐D‐glucanase in barley aleurone

FEBS Letters

Fincher, Geoffrey B.¹  Mundy, John² 

[1] Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia;Department of Biotechnology, Carlsberg Research Laboratory, Garnie Carlsberg Vej 10, DK-2500 Copenhagen Valby, Denmark

关键词: (Barley);    Aleurone;    β-Glucanase;    translatable mRNA;    Gibberellic acid;    Abscisic acid;   

DOI  :  10.1016/0014-5793(86)80434-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyadenylated mRNA has been isolated from barley aleurone layers incubated in the presence and absence of exogenous gibberellic acid and abscisic acid. Immunoprecipitation of in vitro translation products with specific antibodies shows that low levels of translatable mRNA encoding (1 →3,1 →4)-β-D-glucanase 4-glucanohydrolase (EC 3.2.1.73) can be detected in the absence of the phytohormones. Gibberellic acid causes a 10-fold increase in levels of translatable mRNA for the enzyme, while abscisic acid treatment suppresses the relative abundance of translatable (l →3,1 →4)-β-D-glucanase mRNA.

【 授权许可】

Unknown