Mechanism of gene activation by the Escherichia coli positive regulator, OmpR: A mutant defective in transcriptional activation

FEBS Letters

Mizuno, Takeshi¹  Tsuzuki, Masakatsu¹  Kato, Naoki¹  Aiba, Hirofumi¹ 

[1] Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464, Japan

关键词: Positive regulator of E. coli;    Transcriptional activation;    Mutation analysis;    Osmotic regulation;    PCR;    polymerase chain reaction;    bp;    base pair(s);    PC-mutant;    positive control mutant;   

DOI  :  10.1016/0014-5793(94)00846-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The OmpR protein is an activator specific for the E. coli ompC and ompF genes. This protein functions in a phosphorylation-dependent manner through a presumed interaction with RNA polymerase. In this study we isolated OmpR mutants which were suggested to be defective for transcriptional activation, but not for DNA binding. Two such mutants, that we isolated, have a single amino acid alteration at positions 131 [P131S], and 179 [P179L], respectively, of OmpR, comprising 239 amino acids. These altered amino acids in OmpR may be implicated, directly or indirectly, in the presumed RNA polymerase/OmpR interaction that is important for transcriptional activation.

【 授权许可】

Unknown