Spatial and temporal regulation of protein expression by bldA within a Streptomyces lividans colony

FEBS Letters

Kosono, Saori¹  Kataoka, Masakazu³  Tsujimoto, Gozoh² 

[1] Laboratory of Microbiology, Riken, Hirosawa, Wako, Saitama 351-0198, Japan;Division of Pediatric Pharmacology, National Children's Medical Research Center, Taishido, Setagaya, Tokyo 154-8509, Japan;Project Research Center, Mitsubishikasei Institute of Life Sciences, Minami-ooya 11, Machida, Tokyo 194-8511, Japan

关键词: Visualization;    bldA regulation;    Green fluorescent protein;    Streptomyces;   

DOI  :  10.1016/S0014-5793(99)01569-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The bldA gene encodes the only tRNA for the UUA codon that, although dispensable in genes important for primary vegetative growth of Streptomyces spp., is important in genes that serve a regulatory purpose in the differentiation. To investigate this role further, the spatial and temporal expression profiles of the bldA-regulated and unregulated genes within a Streptomyces colony were examined using modified genes for the green fluorescent protein (gfp) as an expression-tag. A comparative study, based on computer-assisted quantitative analysis of the GFP fluorescence, revealed that the presence of TTA codons in gfp results in a temporal delay of translation and, consequently, changed the spatial pattern of the GFP expression within a colony, especially during early differentiation. The delay of GFP expression was undetectable at 60 h post-inoculation. These results provide the first extensive evidence that the bldA does indeed play a significant regulatory role during colony differentiation.

【 授权许可】

Unknown