cDNA cloning, expression and characterization of human prostaglandin F synthase

FEBS Letters

Fukui, Motonari²  Nishizawa, Mikio⁴  Okuda-Ashitaka, Emiko⁴  Nakajima, Tatsuya⁴  Watanabe, Kikuko¹  Ito, Seiji⁴  Suzuki-Yamamoto, Toshiko³ 

[1] Division of Applied Life Science, Graduate School of Integrated Science and Art, University of East Asia, 2-1 Ichinomiya-gakuencho, Shimonoseki, Yamaguchi 751-0807, Japan;Department of Respiratory Medicine, Shiga Medical Center for Adults, 5-4-30 Moriyama, Moriyama, Shiga 524-8524, Japan;Department of Anatomy and Cell Biology, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto, Tokushima 770-8503, Japan;Department of Medical Chemistry, Kansai Medical University, 10-15 Fumizono, Moriguchi, Osaka 570-8506, Japan

关键词: Prostaglandin F synthase;    Human lung;    Aldo-keto reductase;    Prostaglandin D2;    9α;    11β-Prostaglandin F2;    Peripheral blood lymphocyte;    PG;    prostaglandin;    PGFS;    prostaglandin F synthase;    PQ;    phenanthrenequinone;    NB;    nitrobenzaldehyde;    AKR;    aldo-keto reductase;    HSD;    hydroxysteroid dehydrogenase;    PAGE;    polyacrylamide gel electrophoresis;    PBL;    peripheral blood lymphocytes;    Con A;    concanavalin A;    DD;    dihydrodiol dehydrogenase;   

DOI  :  10.1016/S0014-5793(99)01551-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone of prostaglandin F synthase (PGFS) was isolated from human lung by using cDNA of bovine lung-type PGFS as a probe and its protein expressed in Escherichia coli was purified to apparent homogeneity. The human PGFS catalyzed the reduction of prostaglandin (PG) D₂, PGH₂ and phenanthrenequinone (PQ), and the oxidation of 9α,11β-PGF₂ to PGD₂. The k _cat/K _m values for PGD₂ and 9α,11β-PGF₂ were 21 000 and 1800 min⁻¹ mM⁻¹, respectively, indicating that the catalytic efficiency for PGD₂ and 9α,11β-PGF₂ was the highest among the various substrates, except for PQ. The PGFS activity in the cytosol of human lung was completely absorbed with anti-human PGFS antiserum. Moreover, mRNA of PGFS was expressed in peripheral blood lymphocytes and the expression in lymphocytes was markedly suppressed by the T cell mitogen concanavalin A. These results support the notion that human PGFS plays an important role in the pathogenesis of allergic diseases such as asthma.

【 授权许可】

Unknown