Cloning, sequencing and tissue distribution of two related G protein‐coupled receptor candidates expressed prominently in human lung tissue

FEBS Letters

Tsai, Cannit¹  An, Songzhu¹  Goetzl, Edward J.¹ 

[1] Division of Immunology and Allergy, Departments of Medicine and Microbiology-Immunology, University of California, San Francisco, CA 94143-0711, USA

关键词: G protein-coupled receptor;    Cloning;    Expression;    Human lung;    Orphan receptor;   

DOI  :  10.1016/0014-5793(95)01196-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel G protein-coupled receptor, named GPR12A, was cloned by a PCR strategy using degenerate primers designed from sequences conserved among receptors for inflammatory mediators. Screening of a human lung cDNA library with GPR12A as a probe also identified a closely-related cDNA (GPR6C.1) that has been previously reported as GPR4 [13]. GPR12A and GPR6C.1 are 46.1% identical in amino acid sequence, but are less than 33% identical to any other known receptors. Northern analysis revealed that they are expressed prominently in the lung. Although the ligands for GPR12A and GPR6C.1 are unknown, their similarity suggests that they are receptors for ligands of similar or identical chemical nature.

【 授权许可】

Unknown