Differential expression of miRNA between the monolayer and three dimensional cells after ionizing radiation

28th International Conference on Photonic, Electronic and Atomic Collisions

Pan, Dong^1 ; Ren, Zhenxin^2 ; Hu, Burong^2

University of Chinese Academy of Sciences, Beijing

100049, China^1

Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, Gansu

730000, China^2

关键词: Beam irradiation;    Human lung;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/488/15/152007/pdf
DOI  :  10.1088/1742-6596/488/15/152007

来源: IOP

【 摘 要 】

We detect the expression of miRNA in 2D and 3D human lung epithelial cells (3KT). And our primary experimental results showed that more miRNA in 3D 3KT down regulated than in 2D 3KT cells after not only X-ray but also C-beam irradiation using the miRNA chip assay. Meanwhile, X-ray induced more significantly differential expression of miRNA when the relative expression value of miRNA in 3D cells were compared to 2D cells after irradiation.