Molecular cloning of a novel candidate G protein‐coupled receptor from rat brain

FEBS Letters

Song, Z.H.¹  Brownstein, Michael J.¹  Bonner, Tom I.¹  Young, W.Scott¹ 

[1] Labaratory of Cell Biology, Building 36, Room 3A17, NIMH, NIH, Bethesda, MD 20892, USA

关键词: G protein-coupled receptor;    Cloning;    Rat brain;    Striatum;    Retrosplenial cortex;   

DOI  :  10.1016/0014-5793(94)00888-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A PCR cloning strategy using primers designed from sequences selectively conserved among a cannabinoid receptor and two orphan receptors, was used to isolate novel G protein-coupled receptors. rCNL3, a 1.75 kb cDNA encoding a 363 amino acid protein, was isolated from a rat cerebral cortex library. Sequence analysis showed that rCNL3 possesses a number of structural characteristics of G protein-coupled receptors and has 61% amino acid identity (from transmembrane region one through the carboxyl-terminus) with two other candidate G protein-coupled receptors. Therefore, these three receptors may comprise a receptor subfamily with identical or closely related endogenous ligands. Northern and in situ hybridization experiments demonstrated that rCNL3 mRNA is expressed in the rat brain, with a prominent distribution in striatum.

【 授权许可】

Unknown