Functional regulation of metabotropic glutamate receptor type 1c: a role for phosphorylation in the desensitization of the receptor

FEBS Letters

Giacometti, Angelo²  Ciruela, Francisco¹  McIlhinney, R.A.Jeffrey¹ 

[1] Medical Research Council Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, UK;Glaxo Wellcome S.p.A., Via A. Fleming, 37100 Verona, Italy

关键词: mGluR1c;    Phosphorylation;    Desensitization;    Cell surface expression;    Metabotropic;    Dimer;    DMEM;    Dulbecco's modified Eagle medium;    GPCR;    G-protein-coupled receptor;    HEPES;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    IP;    inositol phosphate;    mGluR1c;    metabotropic glutamate receptor type 1c;    PBS;    phosphate-buffered saline;    PVDF;    polyvinylidene fluoride;    Quis;    quisqualic acid;   

DOI  :  10.1016/S0014-5793(99)01547-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phosphorylation and desensitization of metabotropic glutamate receptor type 1c in response to agonist and phorbol esters has been studied. Specific immunoprecipitation of mGluR1c from cells treated with agonist or PMA showed a time-dependent increase in the phosphorylation of a membrane protein with the same molecular weight as the dimeric form of the receptor. Measurements of inositol phosphate production showed a rapid functional desensitization of about 90% after agonist treatment, whereas treatment with PMA caused only a 30% loss in the same time. The extent of receptor phosphorylation following the different treatments paralleled the desensitization of the receptor. These results strongly suggest that phosphorylation of the dimeric form of mGluR1c, as a functionally active form, may play a role in its rapid desensitization.

【 授权许可】

Unknown