Chloroplast ribonucleoproteins are associated with both mRNAs and intron‐containing precursor tRNAs

FEBS Letters

Ohta, Masaru¹  Sugita, Mamoru¹  Nakamura, Takahiro¹  Sugiura, Masahiro¹ 

[1] Center for Gene Research, Nagoya University, Nagoya 464-8601, Japan

关键词: Chloroplast ribonucleoprotein;    RNA processing;    Splicing;    Tobacco;   

DOI  :  10.1016/S0014-5793(99)01390-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tobacco chloroplasts possess five conserved ribonucleoproteins (cpRNPs). To elucidate the function of cpRNPs we analyzed their localization and target nucleic acid molecules in chloroplasts. Immunoprecipitation of the stromal extract and Northern analysis revealed that cpRNPs are associated in vivo with not only various species of chloroplast mRNAs but also intron-containing precursor (pre-) tRNAs. This observation strongly suggests that cpRNPs are involved in RNA processing, including mRNA stability and pre-tRNA splicing.

【 授权许可】

Unknown