A monoclonal antibody specific for snRNPs U1 and U2

FEBS Letters

Lührmann, Reinhard²  Nigg, Erich A.¹  Reuter, Rolf²  Lehner, Christian F.¹ 

[1] Institut für Zellbiologie, ETH-Hönggerberg, CH-8093 Zürich, Switzerland;Max-Planck-Institut für molekulare Genetik, Otto- Warburg-Laboratorium, Ihnestrasse 73, D-1000 Berlin 33, Germany

关键词: U snRNP;    RNA processing;    Splicing;    Monoclonal antibody;    Lupus erythematosus;    Antigenic structure;   

DOI  :  10.1016/0014-5793(86)80564-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A monoclonal antibody (D-5) is described which selectively precipitates snRNPs U1 and U2. The antibody was derived from a mouse immunized with extracts from chick embryonic nuclei. By immunoblotting on either total proteins from purified snRNPs U1–U6, U2–U6 or U1 only, we could demonstrate that the monoclonal antibody cross-reacts with the U1 RNP specific polypeptide A and the U2 RNP specific polypeptide B'', thereby establishing that the two snRNP proteins share at least one epitope. D-5 precipitates snRNPs U1 and U2 from a variety of species, including man, chicken, mouse, rat kangaroo and Xenopus laevis. It will thus be a useful tool for studying structure function relationships of the two snRNP species in different cell systems.

【 授权许可】

Unknown