Functional roles of DExD/H-box RNA helicases in Pre-mRNA splicing

Journal of Biomedical Science

Soo-Chen Cheng¹  Yen-Chi Liu¹ 

[1] Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan, Republic of China

关键词: RNA processing;    Spliceosome;    Splicing;    DExD/H-box RNA helicase;   

Others  :  1219613
DOI  :  10.1186/s12929-015-0161-z

 received in 2015-05-18, accepted in 2015-06-29,  发布年份 2015

【 摘 要 】

Splicing of precursor mRNA takes place via two consecutive steps of transesterification catalyzed by a large ribonucleoprotein complex called the spliceosome. The spliceosome is assembled through ordered binding to the pre-mRNA of five small nuclear RNAs and numerous protein factors, and is disassembled after completion of the reaction to recycle all components. Throughout the splicing cycle, the spliceosome changes its structure, rearranging RNA-RNA, RNA-protein and protein-protein interactions, for positioning and repositioning of splice sites. DExD/H-box RNA helicases play important roles in mediating structural changes of the spliceosome by unwinding of RNA duplexes or disrupting RNA-protein interactions. DExD/H-box proteins are also implicated in the fidelity control of the splicing process at various steps. This review summarizes the functional roles of DExD/H-box proteins in pre-mRNA splicing according to studies conducted mostly in yeast and will discuss the concept of the complicated splicing reaction based on recent findings.

【 授权许可】

   
2015 Liu and Cheng.

【 图 表 】

Fig. 1.

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