The possible mechanism of synergistic effects of ethanol, zinc and insulin on DNA synthesis in NIH 3T3 fibroblasts

FEBS Letters

Crilly, Karan S¹  Huang, Jin-Sheng¹  She, Qing-Bai¹  Kiss, Zoltan¹  Mukherjee, Jagat J¹ 

[1] The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912 USA

关键词: Ethanol;    Zinc;    Insulin;    DNA synthesis;    MAP kinase;    Cyclin;    MAP;    mitogen-activated protein;    EtOH;    ethanol;    Cdk;    cyclin-dependent kinase;    Rb;    retinoblastoma;   

DOI  :  10.1016/S0014-5793(99)01349-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In serum-starved NIH 3T3 fibroblast cultures, zinc (15–40 μM) enhanced both the individual and combined stimulatory effects of insulin and ethanol (EtOH) on DNA synthesis. Zinc, but not EtOH, also promoted the stimulatory effects of insulin on activating phosphorylation of p42/p44 mitogen-activated protein (MAP) kinases. In the presence of zinc, insulin induced premature expression of cyclin E during early G1 phase; EtOH partially restored the normal timing (late G1 phase) of cyclin E expression. The results suggest that zinc and EtOH promote insulin-induced DNA synthesis by different mechanisms; while zinc acts by enhancing the effects of insulin on MAP kinase activation, EtOH may act by ensuring timely zinc-dependent insulin-induced expression of cyclin E.

【 授权许可】

Unknown