Growth factor‐like effects of placental alkaline phosphatase in human fetus and mouse embryo fibroblasts

FEBS Letters

Crilly, Karan S¹  Huang, Jin-Sheng¹  She, Qing-Bai¹  Kiss, Zoltan¹  Mukherjee, Jagat J¹ 

[1] The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA

关键词: Placental alkaline phosphatase;    Insulin;    Fibroblast;    DNA synthesis;    Mitogen-activated protein kinase;    ALP;    alkaline phosphatase;    PALP;    placental alkaline phosphatase;    TNSALP;    tissue non-specific alkaline phosphatase;    IALP;    intestinal alkaline phosphatase;    uPALP;    untreated PALP;    hPALP;    PALP pretreated at 65°C for 30 min;    Ca2+;    calcium;    MAP;    mitogen-activated protein;    p70 S6K;    p70 S6 kinase;    PI3K;    phosphatidylinositol 3′-kinase;    DMEM;    Dulbecco's modified Eagle's medium;    MTT;    (3-4;    5-dimethylthiazol-2-yl)-2;    5-diphenyltetrazolium bromide;   

DOI  :  10.1016/S0014-5793(00)01273-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human placental alkaline phosphatase (PALP) is synthesized in the placenta during pregnancy and is also expressed in many cancer patients; however, its physiological role is unknown. Here we show that in human fetus fibroblasts as well as normal and H-ras-transformed mouse embryo fibroblasts PALP stimulates DNA synthesis and cell proliferation in synergism with insulin, zinc and calcium. The mitogenic effects of PALP are associated with the activation of c-Raf-1, p42/p44 mitogen-activated protein kinases, p70 S6 kinase, Akt/PKB kinase and phosphatidylinositol 3′-kinase. The results suggest that in vivo PALP may promote fetus development as well as the growth of cancer cells which express oncogenic Ras.

【 授权许可】

Unknown