α1‐Antitrypsin can increase insulin‐induced mitogenesis in various fibroblast and epithelial cell lines

FEBS Letters

Crilly, Karan S¹  She, Qing-Bai¹  Kiss, Zoltan¹  Mukherjee, Jagat J¹ 

[1] Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA

关键词: α1-Antitrypsin;    Insulin;    Fibroblast;    DNA synthesis;    Mitogen-activated protein kinase;    AT;    α1-antitrypsin;    PALP;    placental alkaline phosphatase;    MAP;    mitogen-activated protein;    DMEM;    Dulbecco's modified Eagle's medium;    MTT;    3-[4;    5-dimethylthiazol-2-yl]-2;    5-diphenyltetrazolium bromide;   

DOI  :  10.1016/S0014-5793(00)01492-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α₁-Antitrypsin (AT), the archetypal member of the superfamily of serine proteinase inhibitors, inhibits leukocyte elastase activity and thereby can prevent lung damage. Here we show that in fibroblasts from human fetal lung and mouse embryo as well as in certain epithelial cells AT can also enhance the stimulatory effects of insulin on DNA synthesis and cell proliferation. Warming of AT at a moderate (41°C) temperature for a longer time (21 h) or at a higher (65°C) temperature for 30 min before treatment increased its stimulatory effects on both DNA synthesis and activating phosphorylation of p42/p44 mitogen-activated protein kinases. The results suggest that AT may promote regeneration of damaged tissues under pathophysiological conditions which are associated with fever.

【 授权许可】

Unknown