Mechanistic aspects of the relationship between low‐level chemiluminescence and lipid peroxides in oxidation of low‐density lipoprotein

FEBS Letters

Melzi d'Eril, Gian Vico²  Palladini, Giuseppina¹  Albertini, Riccardo²  Passi, Alberto²  Abuja, Peter M.³  De Luca, Giancarlo² 

[1] Institute of Internal Medicine, University of Pavia, P. le Golgi, 27100 Pavia, Italy;Department of Experimental and Clinical Biomedical Sciences, University of Insubria, Via Dunant, 21100 Varese, Italy;Institute of Biochemistry, University of Graz, Schubertstrasse 1, A-8010 Graz, Austria

关键词: Low-density lipoprotein;    Lipid peroxidation;    Low-level chemiluminescence;    Tocopherol;    LDL;    low-density lipoprotein;    CD;    conjugated diene hydroperoxide;    LL-CL;    low-level chemiluminescence;    TocOH;    α-tocopherol;    LOOH;    lipid peroxides;    LOO;    lipid peroxyl radical;    LH;    reactive methylene group of a polyunsaturated fatty acid;    L;    carbon-centred lipid radical;    LC=3O;    triplet carbonyl compounds;    3O2;    triplet oxygen;    PBS;    phosphate-buffered solution;   

DOI  :  10.1016/S0014-5793(99)01217-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study oxidation of low-density lipoprotein (LDL) induced by different Cu²⁺ concentrations was investigated. Lipid peroxidation was assessed by monitoring low-level chemiluminescence (LL-CL), conjugated diene hydroperoxide (CD) and α-tocopherol (TocOH), the major lipophilic antioxidant in LDL. At high Cu²⁺ concentration, LDL oxidation was characterised by CD formation, LL-CL emission and TocOH consumption. At low Cu²⁺ concentration, CD formation was independent of LL-CL and occurred in the presence of TocOH. Thus, two different mechanisms lead to lipid peroxide formation in LDL. The combination of CD assay and LL-CL monitoring makes it possible to distinguish the autocatalytic mechanism of CD formation and that associated with TocOH, found at a high and a low rate of initiation, respectively.

【 授权许可】

Unknown