Protection against reactive oxygen species by NAD(P)H:quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA)

FEBS Letters

Sies, Helmut²  Talalay, Paul¹  Wefers, Heribert²  Komai, Toru² 

[1] Department of Pharmacology and Experimental Therapeutics, The Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA;Institut für Physiologische Chemie I, Universität Düsseldorf, D-4000 Düsseldorf, FRG

关键词: Reactive oxygen species;    Dietary antioxidant;    DT-diaphorase;    Low-level chemiluminescence;    Dicoumarol;    Quinone redox cycling;   

DOI  :  10.1016/0014-5793(84)80290-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Menadione elicits low-level chemiluminescence (λ >620 nm) associated with redox cycling of the quinone in mouse hepatic postmitochondrial fractions. This photoemission is suppressed when the animals are fed a diet containing the anticarcinogenic antioxidant, 2[3]-(tert-butyl)-4-hydroxyanisole (BHA), which leads to a 13-fold increase in NAD(P)H:quinone reductase (EC 1.6.99.2). Inhibition of the enzyme by dicoumarol completely abolishes the protective effect of BHA treatment and leads to higher chemiluminescence, reaching similar photoemission for BHA-treated and control animals. These findings indicate that the two-electron reduction promoted by quinone reductase prevents redox cycling and that BHA protects against reactive oxygen species by elevating the activity of this enzyme.

【 授权许可】

Unknown