FEBS Letters | |
Selective peroxisome degradation in Yarrowia lipolytica after a shift of cells from acetate/oleate/ethylamine into glucose/ammonium sulfate‐containing media | |
Barth, Gerold1  Gunkel, Katja1  Veenhuis, Marten2  van der Klei, Ida J.2  | |
[1] Institute of Microbiology, Technical University of Dresden, Mommsenstrasse 13, D-01062 Dresden, Germany;Laboratory for Eukaryotic Microbiology, Groningen Biomolecular Scienes and Biotechnology Institute (GBB), University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands | |
关键词: Yeast; Microbody; Autophagy; Peroxisome degradation; Mutant isolation; Yarrowia lipolytica; | |
DOI : 10.1016/S0014-5793(99)00513-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have shown that peroxisomes of the yeast Yarrowia lipolytica are subject to specific degradation after exposure of acetate/oleate-grown cells to glucose excess conditions. Electron microscopic analysis has revealed that the peroxisomes were degraded by uptake in the vacuole. Our results suggest that peroxisomes are taken up by macroautophagic processes, because sequestration of individual peroxisomes, which occurs typically at the beginning of microautophagy, was never observed. The observation that a peroxisomal amine oxidase activity is specifically induced by ethylamine was used for the development of a plate assay screening procedure to isolate peroxisome degradation-defective mutants.
【 授权许可】
Unknown
【 预 览 】
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