Yeast Mon1p/Aut12p functions in vacuolar fusion of autophagosomes and cvt‐vesicles

FEBS Letters

Barth, Henning¹  Murén, Eva²  Voss, Christiane¹  Ronne, Hans²  Barmark, Gunilla²  Meiling-Wesse, Khuyen¹  Thumm, Michael¹ 

[1] Institute of Biochemistry, University of Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany;Department of Plant Biology, Uppsala Genetic Center, Swedish University of Agricultural Sciences, Box 7080, S-750 07 Uppsala, Sweden

关键词: Ypt7;    Ccz1;    Vacuolar fusion;    Autophagy;    Vesicle docking;    Yeast;   

DOI  :  10.1016/S0014-5793(02)03456-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we identify Mon1p as being essential for the cvt-pathway and autophagy. Thus, mon1Δ cells are impaired in proaminopeptidase I maturation and homozygous diploid mon1Δ cells do not sporulate. Quantitative autophagy measurements suggest a complete autophagy block. The autophagosomal marker protein GFP-Aut7p accumulates in mon1Δ cells at punctate structures outside the vacuole. Furthermore, proaminopeptidase I accumulates in mon1Δ cells in a proteinase-protected form. Our data demonstrate that mon1Δ cells are defective in the fusion of cvt-vesicles and autophagosomes with the vacuole. Consistent with this, GFP-Mon1p localizes to the cytosol and to punctate structures within the cytosol.

【 授权许可】

Unknown