Anandamide stimulates phospholipase D activity in PC12 cells but not in NIH 3T3 fibroblasts

FEBS Letters

Kiss, Zoltan¹ 

[1] Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA

关键词: Anandamide;    PC12 cell;    Phospholipase D;    Phorbol ester;    Nerve growth factor;    Anandamide;    N-arachidonoylethanolamide;    PLD;    phospholipase D;    PtdCho;    phosphatidylcholine;    PtdEtn;    phosphatidylethanolamine;    PtdEtOH;    phosphatidylethanol;    NGF;    nerve growth factor;    PMA;    phorbol 12-myristate 13-acetate;    AAEE;    arachidonic acid ethyl ester;   

DOI  :  10.1016/S0014-5793(99)00295-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The endogenous cannabinoid arachidonoylethanolamide was previously reported to have no effects on the phospholipase D activity in Chinese hamster ovary cells expressing the human brain-specific cannabinoid receptor, while in mouse peritoneal cells, Δ⁹-tetrahydrocannabinol stimulated this enzyme. In this work, arachidonoylethanolamide (0.1–1 μM) was found to stimulate the phospholipase D-mediated phospholipid hydrolysis in rat adrenal pheochromocytoma PC12 cells, but not in mouse NIH 3T3 fibroblasts. The phospholipase D-activating effects of arachidonoylethanolamide were comparable to those elicited by phorbol ester and nerve growth factor, while arachidonic acid (1 μM) had no effects. The results show that, depending on the cell type, arachidonoylethanolamide can be an activator of the phospholipase D system.

【 授权许可】

Unknown