N‐acylphosphatidylethanolamine‐hydrolysing phospholipase D lacks the ability to transphosphatidylate

FEBS Letters

Hansen, Harald S¹  Petersen, Gitte¹ 

[1] Department of Pharmacology, The Royal Danish School of Pharmacy, 2 Universitetsparken, DK-2100 Copenhagen, Denmark

关键词: Phospholipase D;    N-acylphosphatidylethanolamine;    N-acylethanolamine;    Anandamide;    Transphosphatidylation;    DAG;    diacylglycerol;    DMSO;    dimethylsulfoxide;    FAAH;    fatty acid aminohydrolase;    MAFP;    methyl arachidonyl fluorophosphonate;    NAE;    N-acylethanolamine;    NAPE;    N-acylphosphatidylethanolamine;    NAPE-PLD;    N-acylphosphatidylethanolamine-hydrolysing phospholipase D;    NMDA;    N-methyl-D-aspartate;    PA;    phosphatidic acid;    PBut;    phosphatidylbutanol;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    PEA;    N-palmitoyl-ethanolamine;    PEt;    phosphatidylethanol;    PI;    phosphatidylinositol;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    PLA;    phospholipase A;    PLC;    phospholipase C;    PLD;    phospholipase D;    PMSF;    phenylmethylsulfonylfluoride;    PS;    phosphatidylserine;    St. sp.;    Streptomyces species;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/S0014-5793(99)00861-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The N-acylphosphatidylethanolamine-hydrolysing phospholipase D (NAPE-PLD) generates N-acylethanolamines, including N-arachidonoyl-ethanolamine (anandamide), that may be neuroprotective and analgesic. The properties of NAPE-PLD from rat heart and brain microsomes are investigated and compared to those of other PLDs. NAPE-PLD is inhibited by the fatty acid aminohydrolase inhibitor MAFP in high concentrations (≥100 μM) while PMSF in high concentrations (10 mM) tends to stabilise NAPE-PLD activity. Oleate inhibits NAPE-PLD but the enzyme is not affected by PIP₂, α-synuclein or mastoparan. Furthermore, it is for the first time reported that NAPE-PLD is not capable of catalysing a transphosphatidylation reaction like most other known PLDs.

【 授权许可】

Unknown