Biosynthesis and turnover of anandamide and other N‐acylethanolamines in peritoneal macrophages

FEBS Letters

Schmid, Patricia C.²  Shiota, Yasuo¹  Krebsbach, Randy²  Kuwae, Toyoyasu¹  Schmid, Harald H.O.² 

[1] Faculty of Pharmaceutical Sciences, Josai University, Keyakiday, Sakado, Saitama 350-02, Japan;The Hormel Institute, University of Minnesota, 801 16th Ave NE, Austin, MN 55912, USA

关键词: N-Acylethanolamine;    N-Acyl PE;    Anandamide;    Macrophage;    N-Acyl turnover;    NAE;    N-acylethanolamine;    N-acyl PE;    N-acylphosphatidylethanolamine;    TLC;    thin-layer chromatography;    GC/MS;    gas-chromatography/mass spectrometry;   

DOI  :  10.1016/S0014-5793(99)01226-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyunsaturated N-acylethanolamines (NAEs), including anandamide (20:4n−6 NAE), elicit a variety of biological effects through cannabinoid receptors, whereas saturated and monounsaturated NAEs are inactive. Arachidonic acid mobilization induced by treatment of intact mouse peritoneal macrophages with Ca²⁺ ionophore A23187 had no effect on the production of NAE or its precursor N-acylphosphatidylethanolamine (N-acyl PE). Addition of exogenous ethanolamine resulted in enhanced NAE synthesis by its N-acylation with endogenous fatty acids, but this pathway was not selective for arachidonic acid. Incorporation of ¹⁸O from H₂ ¹⁸O-containing media into the amide carbonyls of both NAE and N-acyl PE demonstrated a rapid, constitutive turnover of both lipids.

【 授权许可】

Unknown