The phorbol derivatives thymeleatoxin and 12‐deoxyphorbol‐13‐O‐phenylacetate‐10‐acetate cause translocation and down‐regulation of multiple protein kinase C isozymes

FEBS Letters

Messing, Robert O.¹  Roivainen, Reina¹ 

[1] Department of Neurology, University of California, and the Ernest Gallo Clinic and Research Center, San Francisco General Hospital, San Francisco, CA 94110, USA

关键词: Phorbol ester;    Protein kinase C;    PC12 cell;    12-Deoxyphorbol-13-O-phenylacetate-20-acetate;    Thymeleatoxin;    Phorbol 12-myristate;    13-acetate;    PMA;    phorbol 12-myristate;    13-acetate;    dPPA;    12-deoxyphorbol-13-O-phenylacetate-20-acetate;    Tx;    thymeleatoxin;    PKC;    protein kinase C;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(93)80031-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phorbol esters such as phorbol 12-myristate,13-acetate (PMA) are potent activators of protein kinase C (PKC), and activate all PKC isozymes except ζ, and λ. Recently, 12-deoxyphorbol-13-O-phenylacetate-20-acetate (dPPA) and thymeleatoxin (Tx) were reported to selectively activate PKCβ₁ (dPPA) and PKCα, -β, and -γ (Tx), but not PKCδ or PKCϵ in vitro. We examined the ability of these phorbol derivatives to translocate and down-regulate PKC isozymes in intact cells. Our findings demonstrate that dPPA and Tx cause translocation and down-regulation of multiple PKC isozymes, including δ and ϵ.

【 授权许可】

Unknown