Differentiation of PC12 cells in response to a cAMP analogue is accompanied by sustained activation of mitogen‐activated protein kinase

FEBS Letters

Tavaré, Jeremy M.¹  Dickens, Martin¹  Young, Stephen W.¹ 

[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK

关键词: MAP kinase;    Cyclic AMP;    Nerve growth factor;    Phorbol ester;    Insulin;    PC12 cell;    NGF;    nerve growth factor;    EGF;    epidermal growth factor;    PDGF;    platelet-derived growth factor;    MAP kinase;    mitogen-activated protein kinase;    ERK;    extracellular signal-regulated protein kinase;    PMA;    phorbol 12-myristate 13-acetate;    CPT-cAMP;    8-(4-chlorophenylthio)-cyclic AMP;   

DOI  :  10.1016/0014-5793(94)80367-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been proposed previously that the sustained activation of mitogen-activated protein kinase may be necessary for the differentiation of PC12 cells. Differentiation of PC12 cells is induced by many extracellular agonists including nerve growth factor (NGF) and cyclicAMP analogues, but not epidermal growth factor (EGF), insulin or phorbol esters. Our results demonstrate that: (i) 8-(4-chlorophenylthio)-cyclicAMP (CPT-cAMP) activates MAP kinase; this raises the possibility that the MAP kinase pathway may be activated by agents that act through adenylate cyclase; (ii) NGF and CPT-cAMP as well as phorbol esters promote sustained activation of MAP kinase. This suggests that while sustained MAP kinase activation may be associated with differentiation it may not be sufficient, and that other as yet unidentified parallel pathways may be involved.

【 授权许可】

Unknown