Phorbol 12‐myristate 13‐acetate (PMA) increases the expression of the nerve growth factor (NGF) gene in mouse L‐929 fibroblasts

FEBS Letters

Houlgatte, Rémi¹  Grogan, Donal Mac¹  Wion, Didier¹  Brachet, Philippe¹ 

[1] INSERM U 298, Centre Hospitalier Régional et Universitaire F-49033 Angers Cedex, France

关键词: Nerve growth factor;    Phorbol ester;    Serum;    mRNA;    Protein kinase;    Protein synthesis;   

DOI  :  10.1016/0014-5793(90)80149-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rise of the NGF mRNA pool which takes place following exposure of L-929 fibroblasts to serum was prevented in the presence of 5 μM K-252a, a compound which inhibits several species of protein kinase activities. To characterize further this phenomenon, L-929 cells growing in a serum-free medium were exposed to cyclic nucleotide analogs, to a divalent cation ionophore or to the phorbol ester PMA. Only this latter compound induced an enhancement of the NGF mRNA pool, suggesting an involvement of protein kinase C in the upregulation of the NGF transcripts. The effects of PMA or serum also require a synthesis of protein since the level of NGF transcripts remained stable in the presence of cycloheximide.

【 授权许可】

Unknown