The protein kinase C activator phorbol‐12‐myristate‐13‐acetate enhances cyclic AMP accumulation in pheochromocytoma cells

FEBS Letters

Ukena, Dieter¹  Daly, John W.¹  Hollingsworth, Elizabeth B.¹ 

[1] Laboratory of Bioorganic Chemistry, Bldg 4, Room 212, National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA

关键词: cyclic AMP;    Phosphatidylinositol;    Phorbol ester;    Nerve growth factor;    Forskolin;    Adenosine;   

DOI  :  10.1016/0014-5793(86)80227-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC₅₀ value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin response and, in addition, caused a slight increase in the potency of forskolin. PMA also enhanced the maximal cyclic AMP accumulation produced by 2-chloroadenosine, and caused a slight increase in potency of the adenosine analog. Since PMA mimics the effect of diacylglycerols that form during the turnover of the membrane lipid, phosphatidylinositol, the results suggest an interrelationship between the systems involved in phosphatidylinositol turnover and cyclic AMP generation in PC 12 cells.

【 授权许可】

Unknown