The activity of the Na+/H+ antiporter in cultured cardiac cells is dependent on the culture conditions used

FEBS Letters

Lazdunski, Michel¹  Frelin, Christian¹  Green, Richard D.²  Vigne, Paul¹ 

[1] Centre de Biochimie, Centre National de la Recherche Scientifique, Parc Valrose, 06034 Nice Cedex, France;Department of Pharmacology, University of Illinois College of Medicine, Chicago, IL 60612, USA

关键词: (Cardiac cell);    Na+/H+ antiport;    Amiloride;    Phorbol ester;    Phosphatidylinositol;    Cell culture;    EIPA;    ethylisopropylamiloride;    TPA;    phorbol-12-acetate-13-myristate;    BCECF;    2;    7-biscarboxyethyl-5(6)-carboxyfluorescein;    PI;    phosphatidylinositol;    PIP;    phosphatidylinositol 4-phosphate;    PIP2;    Phosphatidylinositol 4;    5-bisphosphate;   

DOI  :  10.1016/0014-5793(86)80234-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When chick cardiac cells are maintained in serum-free NCTC-135 medium, the pH_i dependence of the Na⁺/ H⁺ exchanger is much more acidic (pK = 7.0) than when a serum-free M199 medium is used (pK > 7.5). Phorbol esters activate the Na⁺/H⁺ exchanger and produce a cell alkalinization in cells maintained in NCTC-135 medium by shifting the pH_i dependence of the system to more alkaline values. They have no action on cells maintained in M199 medium. The alkaline pH_i dependence of the Na⁺/H⁺ exchanger and the loss of responsiveness of phorbol ester action in M199 medium correlate with increased rates of phosphatidylinositol turnover.

【 授权许可】

Unknown