H+‐translocating ATPase and Na+/H+ antiport activities in the plasma membrane of the marine alga Platymonas viridis

FEBS Letters

Balnokin, Yurii V.¹  Popova, Larisa G.¹ 

[1] Laboratory of Salt Exchange and Salt Tolerance, Plant Physiology Institute, Russian Academy of Science, Botanicheskaya 35, 127276 Moscow, Russia

关键词: H+-ATPase;    Na+/H+ antiport;    Marine alga;    Plasmalemma;    Platymonas viridis;    ΔpH;    electrochemical H+ potential difference;    CCCP;    m-chlorocarbonylcyanide phenylhydrazone;    DTT;    dithiothreitol;    EGTA;    ethyleneglycol-bis(β-aminoethyl ether) N;    N;    N′;    N′-tetraacetic acid;   

DOI  :  10.1016/0014-5793(92)80801-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proton transport by the vanadate-sensitive ATPase in plasma membrane (PM) vesicles from the marine unicellular microalga Platymonas viridis was investigated. The ATP-dependent generation of ΔpH across the membranes of PM vesicles was followed by the changes in the absorbance of the aminoacridine probe. Acridine orange, Na⁺ caused the decay of ΔpH generated by the ATPase, the rate of the decay being dependent on the concentrations of Na⁺ added. The phenomenon was specific for Na⁺. Amiloride inhibited Na⁺-dependent ΔpH decay. The experiments support the idea of a Na⁺-extruding mechanism (H⁺-translocating ATPase plus Na⁺/H⁺ antiporter) operating in the PM of marine alga Pl. viridis.

【 授权许可】

Unknown