Growth stimulation by bombesin does not involve activation of Na+/H+ exchange in chick embryo otic vesicles in vitro

FEBS Letters

Gillespie, J.I.²  Giraldez, F.¹  Greenwell, J.R.² 

[1] Departamento de Bioquimica y Biologica Molecular y Fisiologia, Universidad de Valladolid, 47005-Valladolid, Spain;Department of Physiological Sciences, The Medical School, Framlington Place, The University, Newcastle upon Tyne NE2 4HH, England

关键词: pH;    intracellular;    Cell proliferation;    Development;    Na+/H+ antiport;    Na+:HCO3 symport;   

DOI  :  10.1016/0014-5793(89)81208-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of the mitogen bombesin on pH_i in cells of the chick otic vesicle was studied using dual wavelength microspectrofluorimetric techniques. The results show that the bombesin did not produce an immediate alkalinization in quiescent otic vesicle cells nor a long-term change in pH_i in vesicles reactivated to grow in culture. The recovery of pH_i from an acid load, caused by an NH₄ pulse, involved both Na⁺/H⁺ exchange and Na⁺:HCO₃ influx mechanisms, neither of which was influenced by bombesin. These observations do not fit with a general model whereby pH_i is a universal signal for DNA replication and cell proliferation.

【 授权许可】

Unknown