Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene

FEBS Letters

Schnurr, Kerstin¹  Kühn, Hartmut¹  Borchert, Astrid¹  Thiele, Bernd J¹ 

[1] Institute of Biochemistry, University Clinics (Charité), Humboldt University, Hessiche Str. 3–4, 10115 Berlin, Germany

关键词: Atherogenesis;    Inflammation;    Lipid peroxidation;    Oxidative stress;    Selenium;    SECIS;    selenocysteine insertion sequence;    PH-GPx;    phospholipid hydroperoxide glutathione peroxidase;    GPx;    glutathione peroxidase;   

DOI  :  10.1016/S0014-5793(99)00221-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

15-Lipoxygenases and phospholipid hydroperoxide glutathione peroxidases (PH-GPx) are counterparts in the metabolism of hydroperoxy lipids and a balanced regulation of both enzymes appears to be important for the cellular peroxide tone regulating the expression of redox sensitive genes. In contrast to lipoxygenases the molecular biology of PH-GPx is less well investigated. In this study we cloned the PH-GPx cDNA from a mouse fibroblast cDNA library and the PH-GPx gene from a mouse genomic library. The gene spans approximately 4 kb which includes 1 kb of 5′-flanking region and consists of seven exons and six introns. The immediate promoter region does not contain a TATA box but there are binding sites for several transcription factors which also occur in the porcine gene. Our investigations provide useful tools for future targeted gene disruption studies.

【 授权许可】

Unknown