RGD‐dependent growth of maize calluses and immunodetection of an integrin‐like protein

FEBS Letters

Labouré, Anne-Marie¹  Mandaron, Paul¹  Faik, Ahmed¹  Falconet, Denis¹ 

[1] Laboratoire de Génétique Moléculaire des Plantes, UMR CNRS 5575, Université Joseph Fourier, CERMO, P.O. Box 53X, F-38041 Grenoble, France

关键词: Plant biology;    Cell wall;    Plasma membrane;    Receptor;    Integrin;    Zea mays;   

DOI  :  10.1016/S0014-5793(98)01634-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When maize calluses are grown in the presence of the RGD peptide, important morphological changes are observed indicating the presence of a likely RGD-binding receptor. Polyclonal antibodies generated against the human β1 integrin subunit, the platelet integrin αIIbβ3 (P23) and antibodies specific for either the β3 platelet chain or the αIIb polypeptide cross-react with glycoproteins in Western blot analyses. Immunoprecipitation assays indicate that this maize integrin-like protein shares structural similarities with the animal αIIbβ3 complex. We also show that AcAt2, a polyclonal antibody raised against Arabidopsis proteins purified on an RGD column, interacts with a maize protein.

【 授权许可】

Unknown