Evidence for specific, high‐affinity binding sites for a proteinaceous elicitor in tobacco plasma membrane

FEBS Letters

Blein, Jean-Pierre¹  Wendehenne, David¹  Binet, Marie-Noëlle¹  Ricci, Pierre²  Pugin, Alain¹ 

[1] Unité associée INRA/Université de Bourgogne 692, INRA, BV 1540, 21034 Dijon Cedex, France;Station de Botanique et Pathologie Végétale, BP 2078 Antibes Cedex 06606, France

关键词: Nicotiana tabacum;    Cryptogein;    Elicitor;    Plasma membrane;    Receptor;   

DOI  :  10.1016/0014-5793(95)01108-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Binding of cryptogein, a proteinaceous elicitor, was studied on tobacco plasma membrane. The binding of the [¹²⁵I]cryptogein was saturable, reversible and specific with an apparent K _d of 2 nM. A single class of cryptogein binding sites was found with a sharp optimum pH for binding at about pH 7.0. The high-affinity correlates with cryptogein concentrations required for biological activity in vivo.

【 授权许可】

Unknown