Systemic necrosis induced by overexpression of wheat yellow mosaic virus 14K suppresses the replication of other viruses in Nicotiana benthamiana

Phytopathology Research

Junmin Li¹  Gang Lu¹  Yan Zhang¹  Chuanxi Zhang¹  Jianping Chen¹ 

[1] State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University;

关键词: Systemic necrosis;    Wheat yellow mosaic virus;    14K protein;    Virus infection;    Elicitor;   

DOI  :  10.1186/s42483-022-00122-4

来源: DOAJ

【 摘 要 】

Abstract Systemic necrosis, induced by plant virus-derived elicitors, is considered as one of the most severe symptoms. It has never been reported that the elicitors encoded by wheat yellow mosaic virus (WYMV) can induce systemic necrosis in plant. In this study, we discovered that the WYMV-encoded 14K protein localized to the endoplasmic reticulum (ER) membrane and triggered a necrotic symptom in Nicotiana benthamiana at 5 days post-infiltration (dpi). Meanwhile, overexpression of WYMV 14K suppressed the replication of tobacco rattle virus (TRV) and potato virus X (PVX). Additionally, deletion of the transmembrane domain and substitution of two non-conserved regions in 14K resulted in the loss of the ability of this protein to induce systemic necrosis. Moreover, the 14K homologous proteins of other bymoviruses failed to induce systemic necrosis. Our results, for the first time, reveal that WYMV 14K induces systemic necrosis and suppresses the replication of other viruses.

【 授权许可】

Unknown