In vitro assembly of a β2 cytochrome b 559‐like complex from the chemically synthesised β‐subunit encoded by the Synechocystis sp. 6803 psbF gene

FEBS Letters

Ohad, Itzhak¹  Haehnel, Wolfgang²  Francke, Christof¹  Loyal, Rosemarie² 

[1] Minerva Avron Even-Ari Center for Photosynthesis Research, Department of Biological Chemistry, The Hebrew University, 91904 Jerusalem, Israel;Institut für Biologie II/Biochemie, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D79104 Freiburg, Germany

关键词: Assembly;    Cytochrome b 559;    Photosystem II;    LDS;    lithium dodecylsulfate;    OG;    octylglucoside;    SDS;    sodium dodecylsulfate;   

DOI  :  10.1016/S0014-5793(98)01629-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The α- and β-subunits of cytochrome b ₅₅₉ encoded by the psbE and psbF gene, respectively, are essential components of photosystem II. The exact structure of this cytochrome is not yet known. The β-subunit of the Synechocystis sp. 6803 cytochrome b ₅₅₉ complex was synthesised by means of solid-phase peptide synthesis. Under reducing conditions, two β-peptide molecules could be assembled specifically with one haem to form a β₂ cytochrome b ₅₅₉-like complex. The spectral properties and the midpoint redox potential (48±5 mV) of the in vitro assembled β₂ cytochrome are nearly identical to those of the low potential form of the native cytochrome b ₅₅₉.

【 授权许可】

Unknown