Orientation and nearest neighbor analysis of psbI gene product in the photosystem II reaction center complex using bifunctional cross‐linkers

FEBS Letters

Satoh, Kimiyuki¹  Enami, Isao²  Tomo, Tatsuya¹ 

[1] Department of Biology, Okayama University, Okayama 700, Japan;Department of Biology, Faculty of Science, Science University of Tokyo, Kagurazaka, Tokyo, Japan

关键词: Chemical cross-linking;    Cytochrome b 559;    D2 protein;    Photosystem II;    psbI gene product;    Reaction center;    Spinach;   

DOI  :  10.1016/0014-5793(93)81438-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two distinct cross-linked products containing psbI gene product were generated in the photosystem II reaction center complex from spinach by treatment with bifunctional reagents directed to amino groups. The first product, which was generated by a 3,3'-dithiobis(succinimidyl propionate) treatment, is deduced to be formed between the ϵ-amino group of Lys³ of the psbI gene product, and a side-chain amino group present on the stromal extension of the D2 protein. The CNBr cleavage analysis of the cross-linked product predicted that the amino group of the D2 protein engaged in the cross-linking is either one of the three lysine residues on the N-terminal fragment (from N-terminus to Met¹⁹) or the Lys²⁶⁸ on the 6th fragment (from Val²⁴⁸ to Met²⁷⁵). The second product, which was also generated on the stromal side by a 1,6-hexamethylene diisocyanate treatment preferentially in alkaline conditions, is predicted to be formed between the ϵ-amino group of Lys³ of the psbI gene product and the N-terminal α-amino group of the α-subunit of cytochrome b ₅₅₉.

【 授权许可】

Unknown