Nearest neighbor analysis of D1 and D2 subunits in the photosystem II reaction center using a bifunctional cross‐linker, hexamethylene diisocyanate

FEBS Letters

Satoh, Kimiyuki¹  Tomo, Tatsuya¹ 

[1] Department of Biology, Okayama University, Okayama 700 and Division of Biological Regulation & Photobiology, National Institute for Basic Biology, Okazaki 444, Japan

关键词: Chemical cross-linking;    D1 protein;    D2 protein;    Hexamethylene diisocyanate;    Photosystem II;    Reaction center;   

DOI  :  10.1016/0014-5793(94)00803-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cross-linked product between the D1 and D2 subunits was generated by treating isolated spinach PS II reaction center with a bifunctional cross-linker, 1,6-hexamethylene diisocyanate. To obtain information on the contact site(s) between D1 and D2 proteins, the cross-linked product was cleaved by CNBr, the resultant fragments were separated using a reverse-phase HPLC, and then the partial sequence of the cross-linked polypeptide fragments were determined. By comparing the results with the deduced amino acid sequence of the D1 and D2 proteins from spinach, it is concluded that the C-terminal domains of the D1 subunit (D308-A334) and that of the D2 subunit (Y297-L353) are in close proximity.

【 授权许可】

Unknown