FEBS Letters | |
The effect of herbicides on components of the PS II reaction centre measured by EPR | |
Zimmermann, J.L.1  Rutherford, A.W.1  Mathis, P.1  | |
[1] Service de Biophysique, Département de Biologie, Centre d'Etudes Nucléaires de Saclay, 91191 Gif-sur-Yvette Cedex, France | |
关键词: Herbicide; Photosystem II; Plastoquinone; Pheophytin; ADRY reagent; Cytochrome b 559; EPR; Photosynthesis; Adry; reagents which accelerate the deactivation reactions of the water splitting enzyme; DCMU; 3-(3; 4-dichlorophenyl)-1; 1-dimethylurea; dinoseb; 2; 4-dinitro-6-s-butylphenol; Hepes; N-2-hydroxyethylpiperazine-N-2-ethanesulphonic acid; Mes; 2-(N-morpholino)ethanesulphonic acid; o-phen o-phenanthroline; | |
DOI : 10.1016/0014-5793(84)80161-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Incubation of PS II membranes with herbicides results in changes in EPR signals arising from reaction centre components. Dinoseb, a phenolic herbicide which binds to the reaction centre polypeptide, changes the width and form of the EPR signal arising from photoreduced Q− AFe. o-Phenanthroline slightly broadens the Q− AFe signal. These effects are attributed to changes in the interaction between the semi-quinone and the iron. DCMU, which binds to the 32 kDa protein, has virtually no effect on the width of the Q− AFe signal but does give rise to an increase in its amplitude. This could result from a change in redox state of an interacting component. Herbicide effects can also be seen when Q− AFe is chemically reduced and these seen to be reflected by changes in splitting and amplitude of the split pheophytin− signal. Dinoseb also results in the loss of ‘Signal II dark’, the conversion of reduced high-potential cytochrome b 559 to its oxidized low-potential form and the presence of transiently photooxidized carotenoid after a flash at 25°C; these effects indicate that dinoseb may also act as an ADRY reagent.
【 授权许可】
Unknown
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