Successful transfer of ADA gene in vitro into human peripheral blood CD34+ cells by transfecting EBV‐based episomal vectors

FEBS Letters

Imanishi, Jiro¹  Nakagawa, Masao²  Hirai, Hideyo²  Inaba, Tohru²  Shimazaki, Chihiro²  Satoh, Etsuko¹  Mazda, Osam¹ 

[1] Department of Microbiology, Kyoto Prefectural University of Medicine, Kamikyo, Kyoto 602-8566, Japan;Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan

关键词: Epstein-Barr virus vector;    Hematopoietic progenitor cell;    Gene therapy;    ADA;    adenosine deaminase;    CFU-c;    colony forming unit in culture;    EBNA;    EBV nuclear antigen;    EBV;    Epstein-Barr virus;    PB;    peripheral blood;   

DOI  :  10.1016/S0014-5793(98)01489-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a novel non-viral system for transfecting human immature hematopoietic cells in vitro. Epstein-Barr virus (EBV)-based episomal vectors carrying human adenosine deaminase (ADA) gene cDNA were transfected by electroporation into human peripheral blood (PB) CD34⁺ cells. The transgene-specific mRNA were detected from 37 to 100% of CFU-c (colony forming unit in culture) colonies derived from the transfected cells. A two-fold increase in enzyme activity was also found. These results indicate the successful transfer and expression of genes in human immature hematopoietic cells using the EBV-based episomal vector system.

【 授权许可】

Unknown