A novel role for proliferin‐2 in the ex vivo expansion of hematopoietic stem cells

FEBS Letters

Lodish, Harvey F¹  Tan, Amabel C.L²  Choong, Meng Ling²  Luo, Biao¹ 

[1] Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, USA;Bioprocessing Technology Centre, MD 11 Level 5, 10 Medical Drive, Singapore 117597

关键词: Hematopoietic progenitor cell;    Stromal cell;    Bone marrow;    Fetal liver;    In vitro expansion;   

DOI  :  10.1016/S0014-5793(03)00844-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A family of proliferin genes was discovered on a microarray analysis of hematopoiesis supportive stromal cell lines. Proliferin-2 (PLF2) increased the frequency of long-term culture-initiating cells (LTC-IC) from 1 in 340 to 1 in 256 of the primary hematopoietic stem cell (HSC)-enriched bone marrow cells grown on MS5.1 feeder layer. A repeat using AFT024 feeder layer also showed a similar increase in LTC-IC (from 1 in 386 cells to 1 in 260 cells). The clonogenic output of the LTC-ICs was also increased significantly. The growth of various hematopoietic and stromal cell lines treated with PLF2 was found to increase by 4–27%, as measured by cell count and DNA synthesis assay. These findings open up the possibility of using PLF2 as a new member of the growth factor cocktails for the ex vivo expansion of HSC.

【 授权许可】

Unknown