FEBS Letters | |
Novel repair action of vitamin C upon in vivo oxidative DNA damage | |
Hickenbotham, Peter T1  Mistry, Pratibha1  Griffiths, Helen R1  Hussieni, Amina1  Herbert, Karl E1  Podmore, Ian D1  Evans, Mark D1  Lunec, Joseph1  Mistry, Nalini1  Cooke, Marcus S1  | |
[1] Division of Chemical Pathology, Centre for Mechanisms of Human Toxicity, University of Leicester, Leicester LE1 9HN, UK | |
关键词: Antioxidant; Vitamin C; Antibody; 8-Oxo-2′-deoxyguanosine; DNA repair; DNA damage; | |
DOI : 10.1016/S0014-5793(98)01403-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
There appears to be a paucity of data examining the effect of dietary antioxidants on levels of oxidative DNA damage in vivo, limiting evidence-based assessment of antioxidant efficacy, mechanisms and recommendation for optimal intake. We have examined levels of 8-oxo-2′-deoxyguanosine (8-oxodG) in mononuclear cell DNA, serum and urine from subjects undergoing supplementation with 500 mg/day vitamin C. Significant decreases in DNA levels of 8-oxodG were seen, correlating strongly with increases in plasma vitamin C concentration. Furthermore we established a timecourse for sequential, significant increases in serum and urinary 8-oxodG levels. These results illustrate, for the first time in humans, the kinetics of 8-oxodG removal and processing in vivo, suggesting a role for vitamin C in the regulation of DNA repair enzymes and thereby demonstrating a non-scavenging antioxidant effect.
【 授权许可】
Unknown
【 预 览 】
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