Novel repair action of vitamin C upon in vivo oxidative DNA damage

FEBS Letters

Hickenbotham, Peter T¹  Mistry, Pratibha¹  Griffiths, Helen R¹  Hussieni, Amina¹  Herbert, Karl E¹  Podmore, Ian D¹  Evans, Mark D¹  Lunec, Joseph¹  Mistry, Nalini¹  Cooke, Marcus S¹ 

[1] Division of Chemical Pathology, Centre for Mechanisms of Human Toxicity, University of Leicester, Leicester LE1 9HN, UK

关键词: Antioxidant;    Vitamin C;    Antibody;    8-Oxo-2′-deoxyguanosine;    DNA repair;    DNA damage;   

DOI  :  10.1016/S0014-5793(98)01403-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

There appears to be a paucity of data examining the effect of dietary antioxidants on levels of oxidative DNA damage in vivo, limiting evidence-based assessment of antioxidant efficacy, mechanisms and recommendation for optimal intake. We have examined levels of 8-oxo-2′-deoxyguanosine (8-oxodG) in mononuclear cell DNA, serum and urine from subjects undergoing supplementation with 500 mg/day vitamin C. Significant decreases in DNA levels of 8-oxodG were seen, correlating strongly with increases in plasma vitamin C concentration. Furthermore we established a timecourse for sequential, significant increases in serum and urinary 8-oxodG levels. These results illustrate, for the first time in humans, the kinetics of 8-oxodG removal and processing in vivo, suggesting a role for vitamin C in the regulation of DNA repair enzymes and thereby demonstrating a non-scavenging antioxidant effect.

【 授权许可】

Unknown