Urinary thymine dimers and 8‐oxo‐2′‐deoxyguanosine in psoriasis

FEBS Letters

Evans, Mark D.¹  Bleiker, Tanya O.¹  Hussieni, Amina¹  Cooke, Marcus S.¹  Ahmad, Jabeen¹  Patel, Kayuri¹  Burd, Robert M.¹  Lunec, Joseph¹  Hutchinson, Peter E.¹ 

[1] Division of Chemical Pathology, Centre for Mechanisms of Human Toxicity, PO Box 138, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK

关键词: PUVA;    8-Oxo-2′-deoxyguanosine;    Thymine dimer;    Psoriasis;    Antibody;    Urine;    UVC-poly(dT);    UVC-irradiated polythymidylic acid;    PUVA;    psoralen+ultraviolet A;    T〈〉T;    thymine dimer;    8-OHdG;    8-oxo-2′-deoxyguanosine;    NER;    nucleotide excision repair;    UVR;    ultraviolet radiation;   

DOI  :  10.1016/S0014-5793(99)01402-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Psoralen in conjunction with UVA (PUVA) is perhaps the most effective treatment for psoriasis. It is, however, a risk factor for skin cancer in these patients and there is a need to develop non-invasive assays reflective of treatment-induced DNA damage. We report here the assessment of two important lesions, thymine dimer (T〈〉T) and 8-oxo-2′-deoxyguanosine (8-OHdG), in the urine of psoriasis patients. It was found that, once corrected for urine concentration, the psoriatic group had significantly higher (P<0.0001) urinary levels of thymine dimers compared to the control group. No significant differences in urinary 8-OHdG levels were noted between the psoriatic, atopic dermatitis and control groups. Therefore biomonitoring of therapy from the very start with this simple and non-invasive assay could perhaps be an effective measure of the risk involved with the treatment allowing optimization for minimal-risk therapy.

【 授权许可】

Unknown