Suppression of nitric oxide toxicity in islet cells by α‐tocopherol

FEBS Letters

Burkart, Volker¹  Groβ-Eick, Anne¹  Kolb, Hubert¹  Radons, Jürgen¹  Bellmann, Kerstin¹ 

[1] Diabetes-Forschungsinstitut an der Heinrich-Heine-Universität Düsseldorf, Auf'm Hennekamp 65, D-40225 Düsseldorf, Germany

关键词: Nitric oxide;    Antioxidant;    Vitamin E;    Vitamin C;    Pancreatic islet cell;   

DOI  :  10.1016/0014-5793(95)00401-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show here that preincubation of pancreatic islet cells with α-tocopherol significantly improves their resistance to toxic doses of nitric oxide (NO). No protection was afforded by other antioxidants such as vitamin C or glutathione-monoethyl ester. The pathway of NO induced islet cell death involves DNA damage and excessive activation of poly(ADP-ribose)polymerase leading to irreversible depletion of intracellular NAD⁺. α-Tocopherol was found to interfere at early steps of this pathway, by preventing the occurrence of DNA strand breaks. This indicates that α-tocopherol directly interacts with NO or its reactive intermediates. We conclude that α-tocopherol is not only part of the cellular defence system against oxygen radicals but also protects eukaryotic cells from NO toxicity.

【 授权许可】

Unknown