Synthetic filaments assembled from C‐terminally truncated α‐synuclein

FEBS Letters

Crowther, R.Anthony¹  Spillantini, Maria Grazia²  Goedert, Michel¹  Jakes, Ross¹ 

[1] Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK;Department of Neurology, E.D. Adrian Building, University of Cambridge, Cambridge CB2 2PY, UK

关键词: α-Synuclein;    Filament assembly;    Parkinson's disease;    Dementia with Lewy bodies;    Multiple system atrophy;   

DOI  :  10.1016/S0014-5793(98)01146-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recently two point mutations in the α-synuclein gene have been found in familial Parkinson's disease. The characteristic fibrous neuropathological lesions of Parkinson's and other neurodegenerative diseases have been shown to stain strongly with antibodies against α-synuclein and extracted filaments have been labelled with anti-α-synuclein antibodies. In view of the close involvement of α-synuclein filaments with pathology, it was important to establish an in vitro assembly system. We report here that C-terminally truncated recombinant α-synuclein readily assembles into filaments resembling those isolated from diseased brain and suggest that truncation by proteolysis may play a role in the pathological process.

【 授权许可】

Unknown