FEBS Letters | |
2D‐isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803 | |
Pakrasi, Himadri1  Zak, Elena1  Norling, Birgitta2  Andersson, Bertil2  | |
[1] Department of Biology, Washington University, St. Louis, MO 63130, USA;Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden | |
关键词: Cyanobacterium; Membrane protein; Phase partitioning; Proteomics; Synechocystis 6803; | |
DOI : 10.1016/S0014-5793(98)01123-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Aqueous polymer two-phase partitioning in combination with sucrose density centrifugation offered, for the first time, a 2D-separation method for the isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis 6803 without any cross-contaminations. The purity of the membrane fractions was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. As an initiation of a proteomics project, two prominent proteins, which were observed only in the plasma membrane (Slr1513, a hypothetical protein, and HofG, a general secretion pathway protein), or in the thylakoid membrane (PsaE, a photosystem I protein, and NdhH, a subunit of NADH dehydrogenase), were identified.
【 授权许可】
Unknown
【 预 览 】
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