2D‐isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803

FEBS Letters

Pakrasi, Himadri¹  Zak, Elena¹  Norling, Birgitta²  Andersson, Bertil² 

[1] Department of Biology, Washington University, St. Louis, MO 63130, USA;Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Cyanobacterium;    Membrane protein;    Phase partitioning;    Proteomics;    Synechocystis 6803;   

DOI  :  10.1016/S0014-5793(98)01123-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Aqueous polymer two-phase partitioning in combination with sucrose density centrifugation offered, for the first time, a 2D-separation method for the isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis 6803 without any cross-contaminations. The purity of the membrane fractions was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. As an initiation of a proteomics project, two prominent proteins, which were observed only in the plasma membrane (Slr1513, a hypothetical protein, and HofG, a general secretion pathway protein), or in the thylakoid membrane (PsaE, a photosystem I protein, and NdhH, a subunit of NADH dehydrogenase), were identified.

【 授权许可】

Unknown