Soft x-ray imaging of intracellular granules of filamentous cyanobacterium generating musty smell in Lake Biwa

11th International Conference on X-ray Microscopy

Takemoto, K.^1 ; Mizuta, G.^2 ; Yamamoto, A.^3 ; Yoshimura, M.^4 ; Ichise, S.^5 ; Namba, H.^2 ; Kihara, H.^3

Kansai Medical University, 18-89 Uyamahigashi, 573-1136, Hirakata, Japan^1

Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatus, 525-8577, Japan^2

SR Crenter, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatus, 525-8577, Japan^3

Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, 526-0829, Japan^4

Lake Biwa Environmental Research Institute, 5-34 Yanagasaki, 520-0022, Otsu, Japan^5

关键词: Blue green algae;    Cyanobacterium;    Diameter of granules;    Fine structures;    Fluorescent antibodies;    Green strain;    Polyphosphates;    Soft x-ray microscopy;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/463/1/012052/pdf
DOI  :  10.1088/1742-6596/463/1/012052

来源: IOP

【 摘 要 】

A planktonic blue-green algae, which are currently identified as Phormidium tenue, was observed by a soft x-ray microscopy (XM) for comparing a musty smell generating green strain (PTG) and a non-smell brown strain (PTB). By XM, cells were clearly imaged, and several intracellular granules which could not be observed under a light microscope were visualized. The diameter of granules was about 0.5-1 μm, and one or a few granules were seen in a cell. XM analyses showed that width of cells and sizes of intracellular granules were quite different between PTG and PTB strains. To study the granules observed by XM, transmission in more detail, transmission electron microscopy (TEM) and indirect fluorescent-antibody technique (IFA) were applied. By TEM, carboxysomes, thylakoids and polyphosphate granules were observed. IFA showed the presence of carboxysomes. Results lead to the conclusion that intracellular granules observed under XM are carboxysomes or polyphosphate granules. These results demonstrate that soft XM is effective for analyzing fine structures of small organisms such as cyanobacterium, and for discriminating the strains which generates musty smells from others.