The psbC start codon in Synechocystis sp. PCC 6803

FEBS Letters

Eggers, Beth¹  Carpenter, Shelly D.¹  Vermaas, Wim F.J.¹  Charite, Jeroen¹ 

[1] Department of Botany and the Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, AZ 85287-1601, USA

关键词: Gene;    psbC;    Translation initiation;    CP43;    Cyanobacterium;    Photosystem II;    CP43;    43 kDa chlorophyll-binding protein of PS II;    PS II;    photosystem II;    bp;    base pairs;   

DOI  :  10.1016/0014-5793(90)80085-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The translation start codon for psbC, the gene encoding CP43, a chlorophyll-binding protein of photosystem II, has been identified for the cyanobacterium Synechosystis sp. PCC 6803 using site-directed mutagenesis. An AUG codon, about 50 bases upstream from the end of psbD-I had previously been assumed to be the translation start site of psbC. However, the fact that the AUG codon is not present in psbC from several other organisms, whereas a GUG codon 14 bases upstream from the end of psbD-I is strictly conserved suggests that CP43 translation starts at the latter codon. Mutation of GUG, but not of AUG, led to a loss of CP43 and photoautotrophic growth, indicating that the GUG codon is the sole initiation site for translation of the CP43 protein in Synechocystis sp. PCC 6803.

【 授权许可】

Unknown