Chlorophyll levels in the pigment‐binding proteins of photosystem II A study based on the chlorophyll to cytochrome ratio in different photosystem II preparations

FEBS Letters

Barbato, Roberto¹  Friso, Giulia²  Barber, James¹  Race, Helen L.¹ 

[1]AFRC Photosynthesis Research Group, Wolfson Laboratories, Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK

[2]Dipartimento di Biologia, Universitá di Padova, Via Trieste 75, 35121 Padova, Italy

关键词: Photosystem II;    Chlorophyll-protein;    CP47;    CP43;    Pigment level;    Spinach;    PS;    Photosystem;    cyt;    cytochrome;    SDS-PAGE;    sodium dodecylsulphate gel electrophoresis;    RC;    reaction centre;    UPLC;    high-pressure liquid chromatography;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid;    DM;    n-dodecyl-β-D-maltosiu??;    OG;    n-octyl-β-D-glucopyranoside;    OTG;    n-octyl-β-D-thioglucopyranoside;    chl;    chlorophyll;    pheo;    pheophytin;   

DOI  :  10.1016/0014-5793(91)80947-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The chlorophyll levels in pigment proteins of photosystem II were investigated by using photosystem II preparations with different levels or complexity. Based on the assumption that there is I cytochrome b559 per reaction centre it has been found that oxygen-evolving complexes containing CP26 and CP29 bind 42 chlorophyll molecules, When CP26 and CP29 are stripped away, the resulting PSII cores bind 30 chlorophyll molecules while CP43-less cores bind approximately 18 chlorophylls. It is therefore concluded that CP47 and CP43 bind 9–12 molecules of chlorophyll a and the D1/D2 complex binds 6 chlorophylls. Taken together CP26 and CP29 bind about 12 chlorophyll molecules.

【 授权许可】

Unknown